Are you a biologist (from all branches of life sciences) in India?

Big or small, research or teaching, above school (intermediate) level, but you should be engaged in publishing or designing research methodology. If yes, please share your...

03 April 2016 3,371 1 View

What are feasible ways of improving quality and quantity of Indian science?

The question is linked to the latest nature news (http://www.nature.com/news/indian-elections-fall-flat-on-science-1.15173?WT.ec_id=NATURE-20140508) where it is said that, India currently...

04 May 2014 3,833 4 View

Cytoscape-Visual mapping browser

How we can select number of IDs (~400) simultaneously in discrete or pass through mapping for changing node colour, size or other features?

06 July 2012 6,786 1 View

Co-expression analysis

I am using citrus Affymetrix array to reach a set of genes differentially expressed in particular stages. I would like to know the genes (transcripts) which are co expressed or coregulated....

06 July 2012 7,698 8 View

Science Ethics

In publications, who are authorized to give and have authorship?

06 July 2012 2,885 7 View

Criteria to say that a sequence already exists in databank

Which parameters (based on E values, score, identity or similarity) should we select to demarcate that the sequence we generated in our experiments is new or is present already in any dataset...

05 June 2012 4,187 6 View

Could we read from the 3'-5' end in databanks?

It's very peculiar that we are able to have a BLAST output of the sequence having sequence in 5'-3' direction not in the reverse. What do you think, is the possible reason?

05 June 2012 6,301 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View