What is the optimum concentration to be used for the miRNA mimic and if there is a good working protocol please advice
Hi, I have been working with 3T3-L1 cells for over a year now.However, over the last couple of months they stopped differentiating into adipocytes. I seed at 200k cells/well (12 well plate), and...
23 May 2024 3,356 1 View
Hi, I have a PCR product for DDL gene for Enterococcus faecalis with non-specific binding as appears in the gel every time I run a gel for the positive control I see two bands the first is my band...
29 October 2023 1,315 3 View
I use molecular devices setup to patch acute brain slices, and I face this problem of "overloaded"sign that appears on the amplifier, and even when I try to autocomponsate the pipette it shifts to...
09 August 2023 4,032 3 View
Some genes that resulted from my differential gene expression analysis don't have corresponding ENTREZ ID and I want to perform KEGG pathway analysis but most tutorials analyze data with no...
24 July 2023 3,961 2 View
What is the minimum number of animals in each group used in expermint?
09 July 2023 5,390 3 View
I think of applying the drug as a continuous infusion over 36 hours. I thought of putting it on a bio-compatible membrane over the surface of the brain is this possible? has anyone seen a study...
26 April 2023 8,612 0 View
Hi everyone, I have a problem with my PCR and tried so many trials to get my product..I am working on RAG 1 gene in birds...my PCR doesnt work at all..however..I used many recipes and programs to...
27 October 2022 6,814 3 View
I am following the examples from Schrödinger software but I still face difficulties. It would be nice if I can get help here.
19 May 2022 2,607 0 View
Dears, If there any open source (program) to plot the Nanopartciles and attach ligands. From such structure I would like to calculate the DFT? kind regards
23 March 2022 3,510 5 View
in MTT prepartaion which is more acurate to be dissolved in the media or in PBS ?
14 December 2021 2,169 4 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?
29 July 2024 3,229 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?
23 July 2024 8,059 1 View
Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...
21 July 2024 4,322 1 View
Im trying to transfect A549 cells using lipfectamine 3000. im using a 96 well plate with 1microgram plasmid DNA in each well . should i remove the culture media or change it or decrease it befor...
21 July 2024 7,610 2 View