20 May 2022 0 3K Report

I am following the examples from Schrödinger software but I still face difficulties. It would be nice if I can get help here.

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3T3-L1 cells not differentiating ?

Hi, I have been working with 3T3-L1 cells for over a year now.However, over the last couple of months they stopped differentiating into adipocytes. I seed at 200k cells/well (12 well plate), and...

23 May 2024 3,356 1 View

Transfection of MDA MB 231 with miRNA mimic using lipofectamin 2000 ?

What is the optimum concentration to be used for the miRNA mimic and if there is a good working protocol please advice

17 December 2023 5,683 0 View

PCR non-specific binding?

Hi, I have a PCR product for DDL gene for Enterococcus faecalis with non-specific binding as appears in the gel every time I run a gel for the positive control I see two bands the first is my band...

29 October 2023 1,315 3 View

Overload mark on the amplifier multiclamp 700B ?

I use molecular devices setup to patch acute brain slices, and I face this problem of "overloaded"sign that appears on the amplifier, and even when I try to autocomponsate the pipette it shifts to...

09 August 2023 4,032 3 View

Is it possible to perform KEGG pathway analysis with genes without corresponding ENTREZ ID?

Some genes that resulted from my differential gene expression analysis don't have corresponding ENTREZ ID and I want to perform KEGG pathway analysis but most tutorials analyze data with no...

24 July 2023 3,961 2 View

Hello, is it permissible to use only four rats in each group in the experiment design??

What is the minimum number of animals in each group used in expermint?

09 July 2023 5,390 3 View

Can we put a mebrane above the surface of the dura of brain of rats?

I think of applying the drug as a continuous infusion over 36 hours. I thought of putting it on a bio-compatible membrane over the surface of the brain is this possible? has anyone seen a study...

26 April 2023 8,612 0 View

Do anyone have an idea about why my PCR doesnt work ..and how to tackle this issue?

Hi everyone, I have a problem with my PCR and tried so many trials to get my product..I am working on RAG 1 gene in birds...my PCR doesnt work at all..however..I used many recipes and programs to...

27 October 2022 6,814 3 View

If there any open source (program) to plot the Nanopartciles and attach ligands. From such structure I would like to calculate the DFT?

Dears, If there any open source (program) to plot the Nanopartciles and attach ligands. From such structure I would like to calculate the DFT? kind regards

23 March 2022 3,510 5 View

MTT preparation and PBS?

in MTT prepartaion which is more acurate to be dissolved in the media or in PBS ?

14 December 2021 2,169 4 View

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