We are trying to transfect plasmid DNA into the THP-1 and primary monocytes. However, transfection efficiencies of lipofectamine 2000 and Genjet were not enough to perform subsequent downstream experiments. We are now testing another transfection reagent, FuGene HD, but its transfection efficiency seems to be ~5% by FACS analysis (not yet optimized).
Has anyone compared the transfection efficiencies of FuGene and lipofectamine LTX?
Thanks.
https://www.promega.kr/products/luciferase-assays/transfection-reagents/fugene-hd-transfection-reagent/?catNum=E2311
https://www.thermofisher.com/kr/ko/home/references/protocols/cell-culture/transfection-protocol/transfecting-plasmid-dna-into-thp-1-cells-using-lipofectamine-ltx-reagent.html
Did you achieve satisfactory results for transfection of THP-1 cells?
Thanks
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