Touching Character segmentation Problem..?

More Chandrashekar Gudada's questions See All

Can you send proper recent Journal on Graphene oxide as sole binder in the recycled coars aggregate with fly ash?

Expected Tittle: PERFORMANCE OF FLY ASH BASED RECYCLED COARSE AGGREGATE CONCRETE WITH GRAPHENE OXIDE AS A SOLE BINDER.

22 August 2023 2,670 2 View

How do I crystallize yttrium nitrate hexahydrate?

I have synthesized yttrium nitrate solution from yttria by adding HNO3. Now I want to crystallize it into solid form. I tried doing it the way CuSO4 is crystallized. But I am getting ammonia gas,...

23 November 2021 5,876 3 View

Influence of Multivariate ENSo on Extreme rainfall Events over Coastal Karnataka, India

I analysed trend of the extreme rainfall using 0.25 deg High resolution Daily rainfall Data (HRDRD) of IMD of my study region for the period 1901-2013. The extreme rainfall Indices considered for...

09 August 2017 1,589 1 View

What the recent model available to understand the influence of ENSO with extreme rainfall and daily rainfall events???

Dear Sir/friends, Am working on spatio-temporal variation of rainfall for West coast karnataka, India. Am looking for the identification of modeling for the influence of ENSO, SST and other...

11 April 2017 6,878 2 View

How to calculate the mean frequency and STD. Deviation of the daily rainfall events???

Dear Sir/Friends, Can anyone explain me how to calculate the mean frequency of daily rainfall events and std. deviation for the SW monsoon daily rainfall events. I have extracted the grid points...

08 April 2017 9,641 4 View

To calculate the Convective Available Potential Energy of annual average of Indian monsoon ...???

Dear Sir and Friends, Am new to the reanalysis data, but i understood bit about it. Me working on spatial distribution of rainfall at 0.25deg (25KM) resolution data. So am thinking to map the...

19 March 2017 9,314 6 View

To analyze the anthropogenic factors influencing variability of rainfall in the Coastal region, what are datasets required and its sources?

In the current situation of urbanization, anthropogenic activity, El nino, Landuse cover change and even many other factors are influencing huge variation in spatiotemporal variation of rainfall....

02 November 2016 5,548 4 View

What are the factors influencing spatial variation of rainfall?

Am working on Spatial and temporal variability of rainfall in West coast of Karnataka using IMD gridded data of resolution 0.25 degree. Currently I did trend analysis for my study area and found...

17 May 2016 1,836 7 View

Does the action of HDAC inhibitor (phenylbutyrate) increase total histone levels?

I have treated myocytes with a HDACi ( phenylbutyrate, 5 mM, 48h) and prepared RIPA cell lysates. In WB, together with acetylated, total total histones are also increasing. The levels are basal in...

25 January 2015 2,816 4 View

Which company antibody detects both progesterone A and B receptors?

Hello, I am trying look for progesterone receptor A and B levels across gestation in uterine nuclear extracts by (NuPage) western blotting. I tried antibodies from different companies but nothing...

16 January 2015 7,141 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View

Creating an Automaton/Using Language as the Model?

As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...

31 July 2024 9,859 0 View

Joachim Pimiskern

For example, at first you could give

skeletonization a try.

https://en.wikipedia.org/wiki/Topological_skeleton

Do also search for 'morphological operation'.

Regards,

Joachim

Bruno Martin

It seems to be a difficult problem, made even more difficult for Latin alphabet users because we have no clue what exactly constitutes a letter in your script. If you were to manually label each individual letter with a different color in the image you attached to your post, you might get more precise suggestions from more people (from me at least)!

Bruno