We are using Streptomycetes as the heterologous host for antibiotic production. We need to isolate total RNA from the liquid culture in production media at different time points (2,5 and 7days). RNA will be used for sequencing and qPCR. I have tried Modified Kirby Mix and Triton X-100/EDTA lysis protocol (from here: https://www.ncbi.nlm.nih.gov/pubmed/15177912). However, the integrity of isolated RNA (RIN, Agilent 2100 bioanalyzer) is very low (