Mahmoud Kamal Ahmadi

3 Questions 9 Answers 0 Followers

Questions related from Mahmoud Kamal Ahmadi

Grow E.coli in 384 microplates without shaking?

Hello, I need to grow E. coli in 384 microplates directly from colonies. They contain an Amp resistance plasmid. We cannot shake the plate. I tested LB, but they grow poorly. Any suggestion to...

12 August 2020 5,556 3 View

Problem to remove Kanamycin marker from a Keio strain, any help?

I am working with seven strains of Keio collection, but I have hard time to remove Kanamycin marker from one of those, particularly the one with Delta TyrA. I simply removed kan from the rest six...

19 January 2016 3,808 7 View

Single gene synthesis or whole opron synthesis for protein expression in E.coli?

We need to construct orpon of 6 tandem genes (total 5 kbp) for protein expression in E.coli. We have previously constructed this opron using gnomic DNA and subcloning but we were not pleased with...

17 August 2015 1,366 3 View