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Questions related from Mahmoud Kamal Ahmadi
Hello, I need to grow E. coli in 384 microplates directly from colonies. They contain an Amp resistance plasmid. We cannot shake the plate. I tested LB, but they grow poorly. Any suggestion to...
12 August 2020 5,559 3 View
I am working with seven strains of Keio collection, but I have hard time to remove Kanamycin marker from one of those, particularly the one with Delta TyrA. I simply removed kan from the rest six...
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We need to construct orpon of 6 tandem genes (total 5 kbp) for protein expression in E.coli. We have previously constructed this opron using gnomic DNA and subcloning but we were not pleased with...
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