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Questions related from Mahmoud Kamal Ahmadi
Hello, I need to grow E. coli in 384 microplates directly from colonies. They contain an Amp resistance plasmid. We cannot shake the plate. I tested LB, but they grow poorly. Any suggestion to...
12 August 2020 5,661 3 View
We are using Streptomycetes as the heterologous host for antibiotic production. We need to isolate total RNA from the liquid culture in production media at different time points (2,5 and 7days)....
11 November 2016 5,389 3 View
I am working with seven strains of Keio collection, but I have hard time to remove Kanamycin marker from one of those, particularly the one with Delta TyrA. I simply removed kan from the rest six...
19 January 2016 3,856 7 View
We need to construct orpon of 6 tandem genes (total 5 kbp) for protein expression in E.coli. We have previously constructed this opron using gnomic DNA and subcloning but we were not pleased with...
17 August 2015 1,411 3 View
