How can I activate my splenocite cells from imunized mice?

Hello everyone. We have a vaccine that elicits a protective response against a pathogen in mice but we want to know which is the T helper type that has been developed (Th1, Th2 or Th3). We want...

19 January 2020 4,549 3 View

Determining yield strength using offset requires extensometer?

I would like to know if extensometer is necessary to determine yield strength of nickel 718 alloy? Or using cross head movement to estimate the sample strain is sufficient? I'm using 0.2% offset.

13 January 2019 7,042 7 View

What is the reason for the wide price range of Carbon Nanotubes? How to assess the quality of CNTs?

Suppliers of carbon nanotubes (SWCNT, DWCNT, MWCNT) provide very different quotes for their product which, at an industrial level, should be related with their quality: - How to assess the quality...

05 December 2018 5,514 3 View

Influence of mounted sample height on SEM images?

I have several samples that have been mounted however the sample height differs, which means the working distance will differ as well. May I know how this will influence the the image captured in...

25 March 2018 7,831 4 View

What is the origin of the dots that appear in the SEM pictures of InP or InGaAsP layers after an FIB cut?

I am doing some FIB cuts and analyzing my layer stack. I always see dots in the InP and InGaAsP layers, but I don't know their origin. These dots do not appear when I do a SEM of a cleaved sample,...

27 February 2018 1,151 2 View

Meta analysis in STATA transform OR and RR into logs?

I'm carrying out a meta-analysis on STATA v.13, I'm using the effect size and CI, I transformed them into log scale but some are

03 February 2018 704 4 View

Can you help me with latent class or Latent profile analysis learning materials?

Hi, I want to learn more about latent class/ latent profile analysis as part of a research project. I wish you could point me out to materials/courses based on R or other free software. Thanks.

24 September 2017 8,355 6 View

How to assess motor skills performance?

Estimated community, I am planning to do a behavioural experiment recording fNIRS and muscular activity during (bi)manual motor tasks. These tasks are to be planned in three degrees of complexity,...

20 September 2017 6,387 4 View

The permittivity of Ag is characterized by the Drude model in Comsol Multiphysics

11 August 2017 2,606 2 View

How-to Plot Transmission Spectra vs Wavelength in Comsol

How-to Plot Transmission Spectra vs Wavelength in the Comsol Multiphysics Software?

08 June 2017 2,463 3 View

Are there any open-source datasets containing physiological information about exercises to be used for an OR-model?

I have just recently started a new "weekend project" in addition to my master's studies and I am looking for a data-set. I would like to use some Operations Research to design an optimal gym...

22 February 2021 8,023 1 View

Staining methods for skeletal muscle fiber typing?

does anyone know the best method to differentiate and quantify rapid fatiguing and fatigue resistant muscle fibers? the old method form the 1980's uses ATP staining which is quite labor intense?

21 February 2021 6,483 3 View

Fluo 3-AM in pancreatic beta-cells?

Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...

16 February 2021 453 2 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

Could someone help me troubleshoot my NOS assay protocol for skeletal muscle extracts, please?

I am trying to measure differences in eNOS activity in skeletal muscle in sedentary and exercised mice, following the conversion of 3H-arg to 3H-cit, and assaying the samples with and with out...

31 January 2021 8,324 1 View

What is the best way to minimize/prevent the effects of rigor mortis in excised mouse muscle?

I'm planning to excise mouse muscle to use for ex vivo mechanical testing. What is the best way to prevent the stiffening effects of rigor mortis from confounding results if I cannot isolate the...

30 January 2021 1,641 2 View

Need for help. What goes wrong with my differentiatio process of C2C12?

Hello, I'm now working on C2C12 myoblast cell line. Growing the cells was great, but there are some problems during differentiation process. The cells are not differentiating but frequently...

30 January 2021 9,535 2 View

How to simulate enzymatic hydrolyis of sucrose in a CSTR coupled with ultrafiltration membrane with comsol?

Dear all, I am trying to simulate the enzymatic hydrolysis of sucrose with invertase in CSTR in Comsol. The reaction pathway is such that Sucrose + Water -> Fructose + Glucose. It is desired...

27 January 2021 2,525 4 View

About protocols used for glucose uptake in HepG2 cells?

Hello everyone. I am curently working on a protocol to measure glucose uptake in HepG2 cells using 2-NDBG. The work involves the pre-treatment of cells with an active material and observe if it...

25 January 2021 7,608 3 View

How long does Hela cancer cells take to reach 80% confluency after thawing from liquid nitrogen?

Three days ago, i thawed Hela cancer cells from liquid nitrogen but until now they are still suspended in the media and very very low number of cells became attached to the tissue culture...

06 January 2021 6,511 4 View