Hello everyone,

I am interested to know how I have to calculate the concentration of a specific analyte during the digestion steps (salivary, gastric, intestinal...).

During the method, the sample is dissolved by half between steps, for example: salivary (50%); in gastric step, the salivary step is dissolved by half with the gastric enzyme mix, so the sample is dissolved at 25%... etc etc.

When I obtain my quantification data after LC/MS analysis, how should I express my results? Do I have to take into account the dilution factor for each step? (Saliva x2, Gastric x4 ....)

Kind regards,

José Ángel