To what should I pay attention if I insert a gene after the promoter?

12 December 2017 1 9K Report

If I want to insert a gene AFTER THE PROMOTER of a cloning vector so that I can produce a recombinant protein, what should I pay attention to? Should this gene have a stop codon? How should every primer be? (I use PCR to amplify the gene)

Thanks in advance!

Thanasis Rentis

Hi Sebastian!

Thank you very very much for your suggestions! They helped me a lot!

Actually, these questions I asked, are part of a theoretical exercise for educational reasons in genome compiler. So, I can try whatever I want in this program without fear of ruining a whole experiment!

Best regards!

Thanasis

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