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I am docking a protein structure at different pHs. After obtaining the PDB structure, I protonate the protein at different pHs using pdb2pqr, download the structure, then use Autodock vina to dock...
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Hi all, We have a lentiviral vector with a gene of interest that we would like to express. There is no requirement for stable cell line construction, so we will only be transfecting transiently....
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In most protocols, 50% methanol is used for protein A regeneration, followed by storage, usually in water/buffer/20% ethanol. In my experience, it is critical to apply a 50% methanol wash so the...
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For a project we needed to use a specific device (Bio-Rad Gene pulser) to electroporate mammalian cells. We set up the instrument with the following settings: Voltage = 1.9kV (we had to use this...
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Hello all, I recently started to use peptides for cellular studies. Unfortunately, when I was designing the peptides I did not have a FITC tag to them (the peptide was synthesized by an external...
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Hello, We have been trying to insert peptide tags (
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Hello fellow researchers, I have been attempting to construct a fusion protein plasmid for a while but have yet succeeded. Any suggestions beyond this point would be highly...
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