Leran Mao

4 Questions 12 Answers 0 Followers

Questions related from Leran Mao

Antibody expression - low amounts of heavy chain and full length monomers?

Hi all, I am trying to produce an antibody from a plasmid in Freestyle CHOS cells. The plasmid is a HC/furin/p2A/LC vector. The cells were transiently transfected during the exponential phase and...

13 July 2023 2,390 5 View

Can FITC-labeled peptides be purified other than HPLC?

Hello all, I recently started to use peptides for cellular studies. Unfortunately, when I was designing the peptides I did not have a FITC tag to them (the peptide was synthesized by an external...

01 June 2023 1,624 3 View

No or low protein expression after transfection of a cloned gene- also low A260/230 plasmid ratio?

Hello, We have been trying to insert peptide tags (

08 March 2023 380 6 View

Unsuccessful e coli transformation / incorrect sequence after plasmid extraction?

Hello fellow researchers, I have been attempting to construct a fusion protein plasmid for a while but have yet succeeded. Any suggestions beyond this point would be highly...

21 December 2021 1,391 3 View