we are trying to sort out at which stage the mis-identification of WT vs Tg mice occurred.
how do i set one up? What do I include?
22 July 2024 6,097 4 View
I’m currently working on polyglutamine disease by Sanger sequencing to count the CAG repeats. However, since my targeted genes involve CAT or CAA interruption in the CAG repeats, their...
01 July 2024 8,328 5 View
Is it ok to titrate new antibodies at a low cell concentration like 100,000 cells per 100 uL instead of 1x10^6 cells per 100 uL? I want to titrate four new antibodies at 4-5 different dilutions to...
02 May 2024 7,613 1 View
They say we were cited by an article but we weren't. "Karen, we found a recent article that cited you:Relationship of serum total cholesterol and triglyceride with risk of mortality in...
15 April 2024 3,993 0 View
I have the PCR products of two bacterial genes (gyrA=1024 bp and rpoB=808 bp) and I want to know how many microliters I should add in the agarose gel?.
27 March 2024 2,798 7 View
All working GC Thermo 1310 simple configuration GC FID in first line. Last week error apear when starting the program that Thermo aqusition service not runing. Reinstalation of Win 10 operation...
06 March 2024 6,701 8 View
I measured Cronbach's alpha on the same scale 4 separate times; 3 were in the .80s and one was .46. Why would this be?
30 December 2023 7,677 0 View
Teachers are expected to gain knowledge in pedagogical, content and technological aspects to be effective in the field of teaching. Yet, there are teachers who have a hard struggle to become fully...
06 October 2023 7,580 2 View
Hello everyone, I am doing my undergraduate thesis and I need to make medium strength PDA culture medium. For this, I must mix 19.5 g/l of PDA culture medium plus 7.5 g/l of Bacteriological agar....
20 September 2023 7,923 0 View
Hi, My current research question is looking at whether children at 5-8 months of age have memory. To do this, we have run an eye-tracking study doing 3 encoding slides and 1 test slide. in the...
13 September 2023 3,335 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View