we are trying to sort out at which stage the mis-identification of WT vs Tg mice occurred.
I am working with the NL-G-F and 5x FAD mice. Are there any sequencing databases publicly available to mine for brain or retina? Thanks!
20 January 2021 6,425 1 View
For the past few months, I have been troubleshooting a CRISPR KO of human CD3e in human primary T cells. I got the KO to work one time using 1250 ng TrueCut Cas9 (Thermo) + 250 ng (or 7.5 pmol)...
11 January 2021 6,016 3 View
How do you use Remote sensing and GIS in transportation, what are the types of images you will require, what will be their wavelength, what type of sensors will you require?
20 December 2020 6,375 5 View
Hi, I am interested in non-biological recognition elements for bacterial sensors. I am aware of molecularly imprinted polymers but I do not seem to find many other alternatives. I know that many...
18 September 2020 5,894 3 View
I am analysing treatment group differences at time 2 (two time points only) data on a psych measure outcome (with five covariates: age, gender, CVD, BMI and F2s), and I am interested in one of the...
25 August 2020 6,904 3 View
Hello! Does anyone has a effective way to autoclave 10% skimmed milk without caramelization? I tried 121C for 15min (in the powder form) but I am struggling with the color which is undesirable....
30 June 2020 4,601 3 View
Hi everyone, I am coating stainless steel with 10%PLA and Nanoparticles, and I am trying to find out techniques that I can use to characterize the coating surface aside from SEM and to quantify...
08 June 2020 2,293 7 View
We want to run 10x Genomic Visium spatial transcriptomics on lung tissue from COVID-19+ patients. How can we inactivate the virus so it's safe to work with the tissue at BSL2? The first step is...
28 April 2020 4,704 3 View
I am trying to do for my thesis a uniform homogeneous coating with PLA (polylactic acid with 1.05 dl/ g viscosity) in Stainless steel 316L medical grade via spin coating, but I haven't been able...
07 April 2020 8,556 3 View
I want to determine the phylogenetic age of the members of the Lepidoptera (Sphingidae) based on mtDNA, using BEAST software; But I do not have a good indicator. Please what is mutation rate in...
28 March 2020 1,559 3 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...
27 February 2021 428 3 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...
23 February 2021 7,701 2 View