This is regarding Comet assay.. What is the cause for uneven migration (Directional) of DNA comet ?

01 June 2022 1 761 Report

As per the standard protocol set of comet assay, we had prepared the slides and done the Lysis procedure for 1hr.. later horizontal electrophoresis for 30 min in stationary phase and 30 min run..for 300mA and 25V.

But still all the DNA comets are not moving in one direction, their direction is not fixed , what may be the cause or is it ok.. to analysis the same..

Blood sample: Chronic kidney disease (CKD) patients

Dr.Kiran Nikam

Asst,Prof,

BIMS, Belagavi

Malcolm Nobre

Hello Dr Kiran Nikam

You may have to change your lysis and electrophoresis buffer. Make fresh buffers. Some positively charged molecules on DNA could be causing movements in different direction.

Best.

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