Recently, I cannot use thin-film hydration method to successfully prepare my DPPC MLV. I am sure I didn't change any parameters or steps in my protocol. However. instead of acquiring thin-film, I got transparent aggregation (see attached picture) at the bottom of round bottom beaker after rotavap overnight. The lipid and chloroform I used were purchased two weeks ago so I don't think it is due to the outdated chemicals. I ever doubt rotovaper problem since I found that I could easily pull out the connected beaker even at very high vacuum degree (6mbar). Then I connected a pressure gauge to the rotovaper to test if the rotovaper works well. I tested twice and the gauge pressure very corresponded to the value read from the rotovaper screen. Now, I am so confused, could someone give me any possible explanations about why? Many thanks!
Robert Adolf Brinzer Kangdi Sun after rotary evaporation is it necessary to dry film further in vaccum or flushing with nitrogen gas?
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