ELISA kit or TBA method with spectrofotometric detection?
I have no idea which is the "basal" level of MDA in bovine specie?
Hi everyone, I'm isolating microglia from P1-2 mice and seeding microglia cells from 3 pups brains/T-25 flask. After seeding, I let this astrocytes-microglia mix culture grow for approx 2-3 weeks,...
06 October 2020 3,916 3 View
I need to test the stimulation production of TNF alpha in the RAW cell line by LPS. I am looking for an easy and cheap experiment to quantify the serial dilution stimulation in this macrophage...
27 September 2020 5,366 6 View
Hello! I am looking for good antibodies that help in clearly distinguish between D1- and D2-type medium spiny neurons (MSNs) in the striatum, by immunohistochemistry and immunofluorescent...
31 May 2020 5,009 3 View
Hello! Do you know some good antibodies, suitable for IFL, for differently staining the M2 and M2 microglia phenotypes in rat tissue? I am searching for antibodies specific for RAT because all the...
27 October 2019 7,260 3 View
I need to buy analytical standards for grayanotoxin I, II and III. I only find the type I. Roberta Merlanti
15 May 2019 2,628 4 View
Thanks!
06 October 2016 4,648 3 View
Tetrakis Hydroxymethyl Phosphonium Sulphate and methanogenic bacteria
21 September 2014 8,375 2 View
I have to use 3-MA in my cell culture at high concentration. II know that 3-MA is not very soluble in water, so the solution would be solubilize 3-MA in DMSO, but I know that DMSO generally...
08 June 2014 1,658 3 View
Do more people use cDNA, siRNA, mRNA or miRNA? Would love to hear what people are using?
29 January 2014 1,147 7 View
cDNA, mRNA, siRNA, miRNA
27 January 2014 4,591 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...
25 February 2021 7,698 3 View
Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...
11 February 2021 3,292 3 View
Hi all Scientists; I have similar question. Can you please help me in selecting unique/distinct peptides in case of LC-MS/MS analysis? I have done salivary gland proteomes for...
26 January 2021 7,488 3 View
I stored small pieces of rat colon in PBS at -20 and now I want to do SOD and MDA assay, I also need to do the Bradford assay to quantify the protein content in the samples. can anyone tell me...
07 January 2021 2,488 1 View
I know Vitamin C is unstable and tends to be oxidized quickly. The problem here is I made a Vitamin C beverage at pH 4-5, and it starts to generate gas in the packaging, especially at high...
13 December 2020 6,055 1 View
Hi all, I'm working on RAB10, which is a small RabGTPase and is geranylgeranylated at his c-terminal cysteine residues. This is important for membrane insertion. I'm wondering if this...
04 December 2020 4,026 1 View
hello! I want to extract vesicles from MDA MB 231 cell ligne, culture in serum free media for 48h, would you help me with th number of cells required to have the vesicles? thank you
03 December 2020 6,488 2 View
Hey! I need help on peptide (22 aa) oxidation.This peptide has no methionine, 1 tryptophan & 1 tyrosine. also has 4 cysteine so I used 2 protection groups (trt & acm). For ACM removal, I...
03 December 2020 3,225 2 View
I want to reduce the disulfide bridge in my 7kDa protein. My protein has only 1 cysteine at the surface and thus makes a homodimer using a disulfide bridge. I want to reduce the disulfide bridge...
24 November 2020 3,211 5 View