The thymine in the DNA is longer than they uracil in RNA . Why ?

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How to convert a Text File to a Fasta Format File?

10 November 2018 3,739 0 View

Which came first, the German Peasants War or the English Peasants Revolt?

German Peasants War, English Peasants Revolt

05 June 2018 6,871 1 View

Why the DNA considered a genetic material ?

DNA , genetic material

04 May 2018 9,569 0 View

Which is the most credible free online IQ test?

credible free online IQ test

04 May 2018 3,451 1 View

Are there any animals that actually smile when they're happy ?

animals that actually smile when they're happy

04 May 2018 9,615 0 View

Is an offline IQ test more reliable than a online IQ test?

offline IQ , online IQ test

04 May 2018 2,465 1 View

At the age of 28, is it too late to start a PhD in the biotechnology field or other ?

the age of 28, start a PhD

03 April 2018 3,698 98 View

What is the best way to extract DNA from fungi ?

extract DNA, fungi

02 March 2018 4,759 3 View

Staying in space for a long time affects DNA or gene expression ?

space , DNA, gene expression

02 March 2018 6,469 0 View

If the bacteria multiply all their genes within 36,000 minutes, how many genes have multiply within one hour ?

gene multiply

02 March 2018 4,612 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View