The subjective method involves scoring of colour intensity of stains on micrographs using adjectives like very strong, strong, moderate, feeble and negative and representing these adjective by numbers ranging from 4 to 0 in that order. With this, I feel strongly that analysis of just a micrograh could be different among three individuals. Software imaging method is very expensive and in fact is unavoidable for me.
Derek A Mann
Blinded manual counting of positive stained cells is the best approach.
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