The subjective method involves scoring of colour intensity of stains on micrographs using adjectives like very strong, strong, moderate, feeble and negative and representing these adjective by numbers ranging from 4 to 0 in that order. With this, I feel strongly that analysis of just a micrograph could be different among three individuals. Software imaging method is very expensive and in fact is not affordable for me.
You are correct that imaging software is more accurate. Try Image J, its free and works on many platforms
http://en.wikipedia.org/wiki/ImageJ
http://rsb.info.nih.gov/ij/download.html
Thank you very much sir. I have Image J but it could not analyse colour intensity on micrographs for me. Could it be that I am naive as to theoperation of the software?
To analyse color intensity you need to split the channels. There is a good description of the details here
http://www.fmhs.auckland.ac.nz/sms/biru/_docs/Colour_Analysis_Tools_in_ImageJ.pdf
Honestly sir, this will do the work. But the instructional material is difficult to understand without the plugins downloaded and I am not finding it possible to download the plugins here.Is there a way of helping me with the plugins sir?
My system tells me that Windows needs a programme to open the files. What programme is needed please to open the files?
You can use ImageJ. It is free, straightforward and give good analysis in terms of cell count, area, color intensity, etc.
It has many advantages over Photoshop like when u want to differentiate between cells and fibers having same color, with different shape.
You can download from the following link and the user manual is attached as well.
http://imagej.en.softonic.com/
Can I get the plugins too downloaded from this link? Thanks very much..
MBF image J has all microscopy-related plugin, please have a try! :) https://www.macbiophotonics.ca/software.htm
I have long used a free software "Scion Image".
First You should capture the image and then you turn it in shades of gray.
The scanned image can be processed with this software
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