What is a good reference on designing experiments to determine molecular mechanism of disease or biological process?

I need to learn techniques and strategies in planning experiments that try to figure out the molecular mechanisms. Thanks.

01 January 2021 2,525 3 View

Has anybody had staining problems with lysotracker in live cells?

I am working with two fish cells types, a zebrafish liver cell line and primary cultures of trout macrophages. We are using lysotracker to stain them in order to visualize lysosomes in confocal...

16 December 2020 3,935 2 View

Is TRIS-EDTA-CITRATE buffer (pH 7.8) a good antigen retrieve for the TUNEL assay?

Hello everyone! I performed a TUNEL assay simultaneously with an immunofluorescence (SOX9 antibody) on the same sections of human testis. The fluorescence of TUNEL turned out well, but that of...

15 December 2020 1,270 3 View

Does a manager's background such as culture and attitude influence the performance of employees?

30 November 2020 1,585 2 View

If I want to use mean as calculation for my questionnaire, should I test the reliability and validity?

I'm using questionnaire for my research and mean (average) as my data analysis. I'm wondering how to calculate the reliability and validity of my data.

28 September 2020 3,517 3 View

How to induce with L-arabinose Chaperone Competent Cell BL21 (Takara)?

I am using Chaperone Competent Cell BL21 from Takara, pGro7/BL21 , but it is not clear on the protocol how and when I should induce the expression of the chaperones with L-arabinose. First time I...

14 September 2020 8,488 3 View

Is it better to use the arithmetic mean or geometric mean when averaging MPN?g or CFU/g measurements?

This data is from a microbiological evaluation of streetfood locally sold in our region. Thanks.

12 August 2020 5,981 3 View

Any recommendations on the software to do text mining or sentiment analysis on Chinese reviews?

I am trying to do text mining on Chinese reviews. I have tried out many softwares, like the RapidMiner, Chinese Text Analytics, Python. Most of them seem to require certain level of programming...

12 August 2020 9,109 8 View

Where can I find a good set of microbiological standards for aerobic plate count and coliform levels for street food or ready to eat food?

I need these to evaluate the data I obtained from my study. Thanks

10 August 2020 7,466 12 View

What is a good Thomson Reuters or Scopus indexed journal where I can publish my paper regarding Microbial safety aspects of street foods?

I need a journal with a faster turn around time, reasonable review process, and preferably no publication fees.

15 July 2020 8,241 3 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Is there a recitation score bank system for fair evaluation and assessment of student recitation?

In actual and virtual class, even if there is rubric for recitation, students may still fail to receive fair grades or scores. Others are active in recitation and others are not or others may fail...

26 February 2021 8,387 3 View