Can we incorporate unnatural amino acids into protein using software for graphical image purpose?

I am looking to incorporate a unnatural amino acids which is not available in the swisssidechain database into a protein crystal structure of pdb? I am using chimera 1.14 vis. tool?

26 January 2021 4,064 3 View

I was running Gaussian 09W Programmer in Frequency analysis but Chk File is not opened ?

gaussian frequency analysis was completed but chk file not open, Alpha MO energies Missing in view of the display.

24 January 2021 1,529 2 View

How to obtain the mobility from the EIS data?

Electrochemical impedance spectroscopy

09 August 2020 7,608 2 View

Hi, I would like to sense the Fe(III) in water, therefore how can I overcome the pH interference?

when adding the Fe compound in the water it gets deprotonated and from strong acid. 1.adding base leads to salt formation 2.adding buffer solution leads to Fe concentration change.

11 March 2020 1,785 3 View

Can anyone please suggest some curent research topics in Concrete Technology ?

concrete technology, materials

10 November 2019 8,423 12 View

Whether soil inorganic carbon sequestration leads to land degradation?

Dear senoirs friends, We know that carbon sequestrated as soil organic and inorganic . Several fancy literature reported that IC is high than OC. The organic forms usually labile and available...

22 March 2019 5,606 3 View

NF Kappa B knocked out cell line?

Can anyone please suggest a human cell line with NFkappaB knock out? Will be using it as a control for my experiment. Thanks in adavance.

08 November 2018 9,232 2 View

Santa Cruz Flk1 mAb mouse sc393193 gives me a band at 37kDa, does VEGFR2 ever appear cleaved at that mw?

The molecular weight that the bands should show up is at 150 kDa, however a western blot of several cell lines reveal the band at 37kDa uniformly. Could there be any reason why? My literature...

19 May 2018 3,643 2 View

Can I use Laser Displacement Sensor (LDS) to measure strain?

I am building a small scale UTM machine. I have placed a load cell to measure stress. Can I use a LDS(Laser Distance Sensor) to measure displacement which can be ultimately used to measure strain?

22 April 2018 3,192 1 View

Whether non-significant data represented in chart or graphical representation?

22 September 2017 6,417 5 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

How to calculate Z (the number of molecules in a unit cell) for calculating theoretical density?

I am trying to calculate the theoretical density of my ceramics materials. I use the formula below to calculate the theoretical density: Theoretical density = (Molecular weight x No. of molecules...

24 February 2021 3,823 2 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

Open Source IoT Simulators supported by Machine Learning?

Dear Research Gaters, Let me first add context to the question. I am looking to simulate the waste management system and resources of a city. The hardware devices I need to simulate include,...

21 February 2021 2,333 4 View