We could probably use some more information on your experiments. It sounds like you can detect the proteins of interest in your infected cells but not in cells transfected with your pcDNA3.1 vectors. Are you infecting with a replication competent virus or a viral vector to be able to see expression? The promoter should be very strong in most cells except for hepatocytes or derivatives. I would first ensure that your vectors all express in a highly transfectable cell and then monitor your transfection in your cell of interest with EGFP or something stable. You might be getting degradation of these proteins when expressed alone. 

we have positive control in my transfection，the control can express well ，and EGFP can well monitored  after transfection, besides , it does not work when the transfection were changed to another cell, we think the problem is the plasmid ,the sequence of the gene.

Are you saying that your control EGFP does not express when you transfect into "another cell", meaning the one that your trying to similarly express your tagged proteins? There's still too little information to work with here, but if you suspect the expression plasmids, you can either sequence through the promoter and the ORF and/or use an in vitro transcription/translation system (such as TnT) and the T7 promoter to confirm that you have full length ORFs with in frame tags for each.

https://www.promega.com/products/protein-expression/eukaryotic-cell-free-protein-expression/tnt-quick-coupled-transcription_translation-system/