The methode that i know, how to examine amino acid break bound from the protein is by using titration (peptide bound), How to purify amino acid ?

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What are some of the benefits of e-government ?

benefits of e-government in fiji

18 May 2024 9,019 4 View

How to assess the temperature of a river (using satellite image) that is generated from river waste water?

I want to assess the temperature of a river (using satellite image) that is generated from river waste water. Does water surface temperature assessment is enough for this or I need to do anything...

04 December 2022 9,912 2 View

Does anyone had ever tried extract gelatin from fish-scale ?

give me suggestion for the article/journal that you had tried and give best result/yield to obtain gelatin. which article give obvious instruction ?

17 August 2017 9,350 1 View

Biodiversity index (shanon winner) modification to determine enviromental recondition.

In a particular (natural) environment biological diversity can quantified, For example, a 4 X 4 meter square area in a carrot patch has 300 carrot plants, all the same species. It has a very low...

20 May 2017 3,564 5 View

Any GIS appilcations/method that can be used to monitor the regeneration and regrowth of mangroves?

Mangroves GIS

20 September 2016 5,665 9 View

Cosmos in Chaos

I need opinions on this statement based on what you understand.

01 January 1970 219 5 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View