I have mtDNA I would like to run an IBD test on, however the web service is not available anymore and the email suggested for contact is no longer operational. Can anyone provide another program to run the test?
Cheers
You can do a Mantel test (and partial mantel test, dbRDA, etc) pretty easily in the R package "vegan".
https://cran.r-project.org/web/packages/vegan/vegan.pdf
Arlequin can be used for IBD through Mantel test as suggested by Artur Burzynski too.
Thanks everyone for the help and suggestions.
Cheers,
Madi
According to the research of Pan et al. (2023) entitled, “Diagnostic Efficacy of serological Antibody detection Tests for Hepatitis Delta Virus: A Systematic Review and Meta-Analysis,” what...
07 April 2024 3,234 2 View
Hello, I am currently attempting to optimize a protocol for extracting microbial DNA from cotton swabs that were swabbed against fish skin. I am wondering if you have any recommendations for the...
24 January 2024 6,175 0 View
Hi, I have used this system for several years... now suddenly the Unicorn 5.3.1 cannot control the pumps on the AKTA Purifier... Using the AKTA Purifier by itself, the pumps work fine... When...
01 December 2023 1,380 2 View
I need to make this reaction (pictured) with the same final concentration, but it needs to be in a total volume of 7.5ul instead of 25ul. Can someone please help me by walking me through your math...
08 October 2023 4,800 3 View
I have completed several DFPT calculations (IBRION=8) of a metal-organic framework supercell, according to several tutorials, to plot the phonon bandstructure and density of states using phonopy....
13 July 2023 3,799 0 View
I did a Lexical Decision Task using DMDX. For the spc. file (which tells Analyze what to do with the raw data) I specified that responses less than 200ms or more than three standard deviations...
07 April 2023 5,562 2 View
I'm starting a PhD and considering various methodologies for this research and getting a bit bogged down!
15 March 2023 6,370 2 View
I am attempting to determine the phonon density of states (via DFPT method) of a relatively large unit cell (52 atoms) using Phono3py from my VASP outputs. However, attempts to produce even a...
13 March 2023 3,121 0 View
I am currently trying to calculate the vibrational density of states as well as vibrational spectra for an optimized geometry using VASP, but am still new to the program. I have successfully...
08 March 2023 9,393 2 View
I'm isolating small intestine IEL/LPL following a protocol from Jove “Isolating Lymphocytes from the Mouse Small Intestinal Immune System”, from Qui et al. (2018) In brief, the washed intestine...
05 February 2023 1,731 1 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...
23 July 2024 3,490 6 View