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Questions related from Hamza Sohail
How can we correctly recognize the promoter region for a gene, specially for those genomes which are not well established and annotated? I have read on many places that its almost 2000 bp before...
25 July 2019 9,016 4 View
Can we extract DNA and directly use it for qPCR or it is necessary to extract RNA and then convert into cDNA and use?
24 June 2019 8,909 3 View
Hello everyone, How can i find the specific promoter region of a gene in my crop, as it is not identified yet? However is reported in Arabidopsis genome. What could be the strategy?
17 June 2019 1,703 3 View
Hello Everyone, I am digesting a vector with XBA1 and PMI1 restriction enzymes, but the problem is my DNA fragment also contains PMI1 site, and its creating problem in ligation? Even the other...
05 May 2019 8,941 14 View
Hello everyone, i am using GFP as marker for transgenic plant confirmation but the problem is, whenever i check the GFP positive roots, there i also found too many fake/false roots (without GFP)...
02 April 2019 7,606 6 View
I want to determine ABA (abscisic acid) in citrus leaves...and need easy and implementable protocol for this purpose
08 November 2016 2,145 4 View
