What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View

What is the minimum number of studies in each category required for a multiple subgroup analysis to be viable in a meta-analysis?

Cochrane Handbook mentions subgroup analyses are only warranted if there are more than 10 studies (I have 28), and if they are distributed evenly among categories of subgroup. I am wondering if...

17 February 2021 3,467 4 View

How do you interpret multiple meta-regression in STATA?

Please could someone recommend a resource that explains performing meta-regression with multiple moderators. I have read the STATA META ANALYSIS REFERENCE MANUAL, but this only gives examples of...

17 February 2021 7,376 1 View

How many indicators do I need to represent a latent construct?

Hi folks! We did a large survey and planned to use 3 indicators to represent a latent construct (Sense of Place/SoP) in a latent regression model (structural equation modeling). However, one of...

15 February 2021 7,705 3 View

What is the structure of a photon that can describe the interference experiments that reject the wave nature of light?

De Broglie's double-solution postulated a causal model of Quantum Mechanics. Knowledge of the photon structure was needed to model the particle part of the cause of the Double Slit interference...

11 February 2021 6,869 5 View

How do you incorporate a study that used non-parametric analysis into a meta-analysis where majority of studies used parametric?

I am calculating Hedge's g for effect sizes, so I am pulling means, SDs, and Ns, from each study. However, one study did a Mann-Whitney U test, and so reports Mean Rank, Sum of Ranks, U, Z, and r...

31 January 2021 1,182 5 View

How to share a struct between client.c and server.c in Contiki RPL UDP ?

I'm trying to include udp-client.c in udp-server.c to share a struct between them But I keep getting this error udp-server.c:67:1: error: redefinition of autostart_processes In file included...

16 January 2021 5,013 4 View

Do you know any CEFR-annotated learner corpora for writing tasks?

Dear researchers, I have been working on a machine learning algorithm to classify language learners' writing tasks automatically according to the CEFR levels. The aim is to use...

03 January 2021 7,041 2 View

Who are the leading Indigenous academics who have researched and published on STEM teaching and learning?

I'm interested to read what leading Indigenous academics have to say about the intersection between traditional or Indigenous knowledges/epistemologies, values/axiologies and ways of...

14 December 2020 10,026 10 View

How to performe a non-inferiority test in an adjusted linear regression?

I am looking for the relation between weight evolution (continuous) and a binary factor adjusted for confounders. The regression gives an (adjusted) difference and its CI less than the planned a...

13 December 2020 8,061 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View

What is the reason, we got both test and control band on LFA in negative sample?

I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....

11 February 2021 6,558 2 View