Greetings!
Can the hydroxy of water molecule exchange with the carboxyl hydroxy of the Asp side chain in the enzyme? If there are relevant references, please tell me.
Many thanks for your help.
Hi there,
Such exchange 16O /18O is described in the book below (5.3 and 5.4 page 52):
https://books.google.fr/books?id=DmsoDwAAQBAJ&pg=PA53&lpg=PA53&dq=ASP+and+Glu+and+carboxylic+oxygen+exchange+in+protein&source=bl&ots=92hTrWFrg9&sig=ueK_ySXPylttv-qskYpO0l5vJrs&hl=fr&sa=X&ved=0ahUKEwjQ4rrny5fVAhUJa1AKHXLoBucQ6AEIRjAE#v=onepage&q=ASP%20and%20Glu%20and%20carboxylic%20oxygen%20exchange%20in%20protein&f=false
See references 84 to 88 (on page 73 of the book) for detailed protocols.
Dear Dominique Liger,
Thank you very much for your quik response. I'm sorry that I can't access this link. Can you tell me the book name? I'll try to check it.
Thanks a lot.
Best wishes,
Yayue Wang
The book is : Amino Acids, Peptides and Proteins, Volume 40
by Maxim Ryadnov,Ferenc Hudecz
published by the Royal Society of Chemistry.
http://pubs.rsc.org/en/content/ebook/978-1-78262-059-4
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