Hello, in my current experiment, I transfected brainbow system into a mouse cell line, which came from transgenic mice with cre-ert2 recombinase. then I add tamoxifen to check the color change. the result is quite unstable and low efficiency. after add TAM at 2 consecutive days, I found 2 site (about 5 cells in each site) changed color, then i try tamoxifen concentration at 5uM, 20uM. after 48h, i didn't see any color change in these cells. i don't know why the effeciency is so low, and in the future I prepare to injection the cells into mice and treat mice with TAM. This is my first time working with something like this; did anyone can give me some suggestion? Any advice is welcome!

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