Recently, I want to detect the cardiomyocyte cell viability including live cell staining, total cell nuclei staining and apoptosis cell staining. Also, in order to show the function of cardiomyocyte, I need to get the Ca2+ flux, by Ca2+ imaging. For the live cell staining, I want to use the Calcein AM. For the Ca+ imaging, I want to use Rhod-3 Calcium Imaging Kit. Is this double staining suitable? I see the principle of Calcein AM staining and Rhod-3 Calcium are much similar. They will bond to Ca2+ and give off fluorescence signal. Could I just use the Calcein AM to take the Ca2+ imaging? I am beginner of cell staining. So I have several problems.
Hello Yang, Calcein AM does not bind Ca2+. Instead, it is converted by cytosolic esterases into green fluorescent calcein. The latter is now rarely used as a Ca2+ indicator because its fluorescence is directly sensitive to these ions only at strongly alkaline pH.
In the paper below the authors studied programmed cell death modulated by Ca2+ influx with the help of Calcein-AM.
Article Novel fluorescence assay using Calcein-AM for the determinat...
This one is for calcein as Ca2+ indicator.
Article High and low affinity Ca2+ binding to the sarcoplasmic retic...
Hello Yang, Calcein AM does not bind Ca2+. Instead, it is converted by cytosolic esterases into green fluorescent calcein. The latter is now rarely used as a Ca2+ indicator because its fluorescence is directly sensitive to these ions only at strongly alkaline pH.
In the paper below the authors studied programmed cell death modulated by Ca2+ influx with the help of Calcein-AM.
Article Novel fluorescence assay using Calcein-AM for the determinat...
This one is for calcein as Ca2+ indicator.
Article High and low affinity Ca2+ binding to the sarcoplasmic retic...
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