Yang Jiandong

5 Questions 6 Answers 0 Followers

Questions related from Yang Jiandong

How to prepare a 3mM free fatty acid cell culture medium( PA:OA=1:2) ?

Recently, I am planing to use 3mM free fatty acid to treat the HepG2 cells. I am looking for the protocol for this. My protocol: Working solution preparation: (1) Put a final concentration of...

11 November 2018 417 0 View

What kinds of cytokines dose the hepatocyte secrete?

I am a fresh man in cell molecular biology. I was studying the liver metabolites function and the inflammatory response with exogenous toxicant. I knew the hepatocyte is the metabolite center for...

08 August 2018 9,669 7 View

Prevent ethanol losing from cell culture medium by silicone oil ?

Recently, I used different ethanol concentration to treat cells. The ethanol is dissolved in the DMEM cell culture medium. But I found one problem is that the ethanol will evaporate from cell...

06 June 2018 6,680 2 View

The difference between cell viability testing reagent-Calcein AM and Calcium Imaging?

Recently, I want to detect the cardiomyocyte cell viability including live cell staining, total cell nuclei staining and apoptosis cell staining. Also, in order to show the function of...

05 May 2018 475 4 View

Will gene background difference affects the cell-cell junction interaction and recognition?

Recently, I was studying the cell-cell junction interaction. I realized the junction protein of cells have selective connection property. For example, cadherin help selective cell–cell...

01 January 1970 5,367 0 View