Hi all, as described in the title. I isolate genomic DNA, total RNA and total protein from WT and X1 gene KO MEF cell. I am surprised when I know the concentration of the sample. The initial cell number of KO MEF were less than WT MEF, but the samples from KO MEF have more DNA, RNA and protein than WT MEF.
Nilay Mitash
Before answering you question I would like to know
A. what is the function of the gene which is KO?
B. Is it a gene associated with cell cycle?
C. Is it a kinase or phosphatase?
I think you will get your answer while answering these questions.
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Susnata Karmakar
I am intrigued to know how was the quantification of total DNA, RNA or protein done?
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