Nilay Mitash

2 Questions 10 Answers 0 Followers

Questions related from Nilay Mitash

Can we transfect polarized epithelial cells grown on collagen coated inserts with siRNA by adding it directly into basolateral media?

I want to silence a gene in primary cells using siRNA approach. I have already seen effects of miRNA mimics in similar cell line but on different gene by adding them directly into media (7 days...

29 April 2019 9,472 3 View

How to use miRmap Score for indicating repression strength of miRNAs?

I am searching miRmap score for some miRNAs having binding site in 3'UTR of CFTR. I am little bit confused. Example: When I am putting just the name of a miRNA in miRmap application and the gene...

01 January 1970 8,292 1 View