Which search engines can I use to get the source of a certain microbial strain?

I am searching for a certain website through which can I get the source of certain microbial strains, especially probiotic strains I mean a search engine contains a database of the identified...

20 December 2020 5,144 5 View

Can you help me choose a topic to my thesis?

Good Morning, I am a Ph.D. student studying " Information Management & Libraries" interested to work on a topic related to technology and how it is changing the future of libraries, but I...

14 December 2020 3,848 14 View

I need to know my H-index, my citations and publications?

How can I obtain my H-index, citations, publications?

01 December 2020 7,589 6 View

Can I use Scherrer formula to calculate the crystallite size/Coherent domain size of Li2O5Si2 (lithium disilicate) from XRD data?

I`m comparing two different samples containing lithium disilicate and under SEM (5000x) there was a noted increase in the particle size (1st sample 2.7 microns in length and the 2nd sample 5...

28 October 2020 5,905 6 View

Calculating Seeded Cells?

I want to do a cell based assay. This assay contains 5 uL of my cells (Dissolved in DMEM medium) and 5 uL of compound (dissolved in DMED medium also). Suppose I count the cells in my suspension...

19 October 2020 2,487 1 View

Why I am losing the Zona of pellocida while doing mouse preimplementation embryo staining?

I am doing some staining experiments in mice embryos and I noticed that I lose the zona of pellocida during the procedures. I can not define at exactly which step I lose it but mostly either...

15 October 2020 2,906 2 View

How to prepare 0.2 M Sodium Acetate Buffer Solution, pH 3.5?

12 October 2020 9,558 3 View

How to preserve Fe3O4@SiO2@Chitosan nanoparticles for a long time?

Is freeze-drying an appropriate method for these types of nanoparticles? If it's ok, what are the relevent buffers and conditions?

11 October 2020 2,276 3 View

Do you know of any source to buy hemp seeds?

Industrial hemp seeds with a good germination rate

28 September 2020 7,568 3 View

Is there a Deep Learning baseline (preferably written in PyTorch) for predicting animal pose from scratch (not like DeepLabCut or DeepPoseKit)?

I am looking for a animal pose estimation code that predicts the pose of an animal given 100-200 annotated frames from scratch using deep learning on a frame-by-frame basis. Is there any such...

27 September 2020 8,957 3 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Trouble shooting with his tagged protein expression in E.Coli?

i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions

15 February 2021 8,939 3 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View