Is there any latest updated tool For download TCGA cancer dataset ?

Previously "TCGA2STAT" a tool of R is used to download TCGA cancer data and their corresponding normal samples, but this tool is deprecated now. Now I need to download a lot of cancer samples as...

11 November 2020 860 5 View

Can the size of TiO2 powder be redused if it was dissolved in NaOH then precipetated by HCL ??

Can the size of TiO2 powder be becreased if it was dissolved in 2.5 M NaOH, then Precipetated by 1 M conc. HCL, according to the following equations: TiO2 + 2NaOH = Na2TiO3 + H2O Na2TiO3 + 2HCL =...

09 September 2019 3,647 3 View

Can the size of TiO2 powder be redused if it was dissolved in NaOH then precipetated by HCL ??

Can the size of TiO2 powder be becreased if it was dissolved in 2.5 M NaOH, then Precipetated by 1 M conc. HCL, according to the following equations: TiO2 + 2NaOH = Na2TiO3 + H2O Na2TiO3 + 2HCL =...

08 September 2019 4,675 4 View

Which industries produce nano-waste??

Where can I find a list or a survey contains any industrial waste in the nano- form.... in general or those dumped in the environment ?? Thanks in advance..

06 July 2018 7,607 6 View

What does " dried in air atmosphere at about 400oC." mean in a protocol for ZnO nanoparticles prepration?

I'm preparing ZnO nanoparticles using the methodology in a paper titeled ' SYNTHESIS AND CHARACTERIZATION OF ZNO NANOPARTICLES WITH ZINC CHLORIDE AS ZINC SOURCE' but at the drying step they said...

04 May 2018 5,370 0 View

Which is more important for catalysis (electrocatalysis), particle size and/or crystallographic orientation?

07 August 2015 9,049 2 View

Is pyrex glass cell is quite good for electrochemical measurements in alkaline medium?

Some researcher suggested Telfon, but it is expensive and not easy to fabricate everywhere

05 June 2015 9,812 0 View

What is the simplest way to calculate the thickness of thin polymer layer developed on the carbon electrode surface by electrochemical polymerization?

If possible without sophisticated techniques and without destroying the polymer layer itself.

01 February 2015 248 3 View

How can i get the list of impact factor of journals due to Tomson (JCR- Journal Citation Report) in some branch?

I mean some link?

01 February 2015 6,435 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View