I'm designing a construct with iCre. The sequence from the publication describing this modified Cre sequence for the first time (Shimshek et. al 2002, genesis, vol. 25;32;, no. 4;1;, pp. 19-26) differs quite a bit from ones used in recent publications even if this paper is cited for the sequence used. From what I noted some modifications at the start and in the end are common. I would be glad for some input on this topic.
GenBank: AY056050.1
ORIGIN 1 aagcttgtcc accatggtgc ccaagaagaa gaggaaagtc tccaacctgc tgactgtgca 61 ccaaaacctg cctgccctcc ctgtggatgc cacctctgat gaagtcagga agaacctgat 121 ggacatgttc agggacaggc aggccttctc tgaacacacc tggaagatgc tcctgtctgt 181 gtgcagatcc tgggctgcct ggtgcaagct gaacaacagg aaatggttcc ctgctgaacc 241 tgaggatgtg agggactacc tcctgtacct gcaagccaga ggcctggctg tgaagaccat 301 ccaacagcac ctgggccagc tcaacatgct gcacaggaga tctggcctgc ctcgcccttc 361 tgactccaat gctgtgtccc tggtgatgag gagaatcaga aaggagaatg tggatgctgg 421 ggagagagcc aagcaggccc tggcctttga acgcactgac tttgaccaag tcagatccct 481 gatggagaac tctgacagat gccaggacat caggaacctg gccttcctgg gcattgccta 541 caacaccctg ctgcgcattg ccgaaattgc cagaatcaga gtgaaggaca tctcccgcac 601 cgatggtggg agaatgctga tccacattgg caggaccaag accctggtgt ccacagctgg 661 tgtggagaag gccctgtccc tgggggttac caagctggtg gagagatgga tctctgtgtc 721 tggtgtggct gatgacccca acaactacct gttctgccgg gtcagaaaga atggtgtggc 781 tgccccttct gccacctccc aactgtccac ccgggccctg gaagggatct ttgaggccac 841 ccaccgcctg atctatggtg ccaaggatga ctctgggcag agatacctgg cctggtctgg 901 ccactctgcc agagtgggtg ctgccaggga catggccagg gctggtgtgt ccatccctga 961 aatcatgcag gctggtggct ggaccaatgt gaacatagtg atgaactaca tcagaaacct 1021 ggactctgag actggggcca tggtgaggct gctcgaggat ggggactgaa actgagtcga //
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