When the apoptosis of Caco-2 was detected by flow cytometry , the negative control cells die too much, especially the proportion of cells in the upper left quadrant is too large. is there any solution?
Scholars from multiple disciplines stressed the importance of AI Technology in ESL teaching from a theoretical perspective. Are there teaching designs that combine theoretical discussions and...
01 July 2024 999 7 View
Now, solar energy is popular for offshore application. Did anyone knows how to estimate the power performance of PV panel? such as empirical formulas or CFD simulation? Thanks.
26 April 2024 8,372 1 View
Dear all, I encountered a weird situation in my research. I have 3 groups, they are all non-parametric and no equal variance as well. I ran a Kruskal-wallis test to compare the difference and my...
06 February 2024 9,454 4 View
I am aimed to find out the diagnosis-relevant biomarkers by recurrence feature elimination(RFE), and selected random forest and logistic as models to be fit. But the number of selected features...
23 November 2023 9,190 0 View
"In a rectangular coordinate system with the vertex of the conical surface as the origin, the conical surface can be represented by homogeneous equations of x, y, and z" This is a conclusion we...
09 November 2023 3,639 4 View
Hello everyone, I'm Stephen from Chongqing GSC. I joined Maersk for 2 years. In September 2022, I was lucky enough to be selected for the LEAD project. One year later, I graduated from the...
08 November 2023 9,182 1 View
I am PHD student in USM,I am researching about agile management,but I cannot find the scales of agile management anymore,who can send somes about agile management,best appreciation!
02 November 2023 7,229 3 View
Where can I find the standard card for Raman spectroscopy of metal oxides? I searched for a long time but couldn't find it
08 August 2023 8,811 2 View
Is there any regularity in the solubility of electrolyte salts in polymer gels? Taking polyvinyl alcohol (PVA) as an example, LiCl can be well dissolved in PVA, followed by NaCl, and KCl is very...
13 July 2023 8,631 1 View
I am submitting to the IEEE Access journal, but I do not find if it is allowed for co-first author in that journal. If allowed, is there any format that I can follow to designate my co-first author?
19 June 2023 8,764 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View
As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...
31 July 2024 9,859 0 View