Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View

The algal cell always gather together when the cells concentration is high and those cells would block the microfluidic chips. How can i handle it?

I want to pump a high concentration algal cells into microfluidic chip, however those cells are always gather together forming a clusters to block the connection between Teflon tube and...

26 October 2020 5,366 2 View

What kind of small mobile robots are available on market to undertake multi-robot research?

Dear All, What kind of small mobile robots are available in market to undertake multi-robot research? Would you have anything to be recommended? Thanks! Chaomin Luo ~~~ Chaomin Luo,...

04 October 2020 5,173 5 View

Why some structural equation models are all observation variables?

Hello everyone. Recently, when I searched the literature about SEM, I found some problems that I couldn't solve. I found that the models established in some studies are all observation variables...

16 July 2020 3,279 3 View

2D protein gel system?

Want to set up a 2D protein gel system. Can anybody recommend a system to go for? We use a lot of Bio-Rad's protein gel systems, but don't know which one is best for 2D gels.

07 July 2020 6,327 3 View

Is there any open source code for learning in the field of data-driven robust optimization?

I can get a lot of theoretical knowledge in related fields, but I'm still confused about the implementation behind it. Unfortunately, I didn't find any examples on the Internet that I could...

29 March 2020 6,438 3 View

Why the large grain contains small grain inside ?

We currently recieved a sample from industry. It is high manganese steel (Hadfield steel) with a composition of Fe-12Mn-1.1C, after austenization treatment. We find that the grain size of this...

15 January 2020 6,624 1 View

How to read the intersection signal information in the Internet of Vehicles environment?

The existing method can be realized by short-distance traffic through the DSRC, or the signal light device can be connected to the roadside device RSU by wire. Even in the driverless field, the...

18 November 2019 6,706 1 View

Are there recommendations to buy some small mobile robots to test our multi-robot algorithms?

We would like to validate and test our multi-robot algorithms with some research-platformed mobile robot. Are there recommendations to buy some small mobile robots to test our multi-robot...

15 November 2019 5,772 3 View

How should the bone model be considered when screw drilling into bone finite element modeling?

For the establishment of bone model, besides considering the bone density, elastic modulus, poisson's ratio, yield stress and yield strain, should the damage caused by bone being cut by screws be...

09 November 2019 5,831 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View