Hello everyone,
I have been reading about the addition of cap 5' in the maturation process of mRNA in eukaryotic cells and from what I have managed to understand, the addition of cap 5' is added only with the presence of RNA polymerase II, This has a domain that is able to recruit the necessary proteins for the formation of the cap 5' and with this is enough for that process to occur.
Now, I want to get out of doubt, this process does not need a signal sequence in the mRNA to add the cap 5' ?, as is the case of the addition of the poly A tail where the signal sequence is needed "AAUAAA" in the mRNA for polyadenylation to occur.
Thank you very much in advance.
It looks to me like independent cell machineries, those dealing with 5'cap generation and polyA appending after recognizing polyadenylation signal (AAUAAA and variants of thereof).
see this
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&ved=0ahUKEwiBkofhtc_TAhVlIMAKHcvhAQ8QFggvMAI&url=https%3A%2F%2Fwww.boundless.com%2Fbiology%2Ftextbooks%2Fboundless-biology-textbook%2Fgenes-and-proteins-15%2Frna-processing-in-eukaryotes-109%2Fmrna-processing-447-11672%2F&usg=AFQjCNHMX54D9WOOHmoEp_MgoaTzDlZO9g
The addition of the cap 5' needs a signal sequence in the mRNA?. Available from: https://www.researchgate.net/post/The_addition_of_the_cap_5_needs_a_signal_sequence_in_the_mRNA [accessed May 3, 2017].
If you are going to add the cap by in vitro, please, keep it mind the RNA have to have triphosphate group in 5-end. Good luck!
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