Erwin Luis Muñoz Acuña

6 Questions 6 Answers 0 Followers

Questions related from Erwin Luis Muñoz Acuña

Is it better to store antibiotics aliquots (in solution) at -80°C than at -20°C?

Hi I have a question, I was reading some articles about different conditions for storing aliquots of antibiotics for use in culture media. But I would like to ask you, according to your personal...

28 August 2023 5,654 4 View

Should I extract the proteins from extracellular vesicles (EVs) to correlate with the concentration of EVs in my sample, using Bradford's method?

Hi all, I am doing assays with extracellular vesicles (EVs) in my lab and I have read in many articles that relate EVs concentration to protein concentration. For example, in this article...

30 November 2021 4,444 3 View

Which is the AAV2 ITR sequence?

Hello, I would like to know why some vectors contain this AAV2 ITR sequence, I want to remove that sequence together with others of my vector to make the stable transfection with lipofectamine...

16 August 2018 7,588 4 View

The addition of the cap 5' needs a signal sequence in the mRNA?

Hello everyone, I have been reading about the addition of cap 5' in the maturation process of mRNA in eukaryotic cells and from what I have managed to understand, the addition of cap 5' is added...

01 May 2017 2,033 6 View

Is the way in which I pose my constructon okay?

Hello everyone, I'm trying to transfect a bacterial operon to Arabidopsis thaliana. The operon consists of 6 genes and I am thinking of doing two constructos, one containing 4 and another one...

04 January 2017 5,442 2 View

Is the NOS terminator enough?

Hello everyone, I am designing a "constructo" for Arabidopsis thaliana and I have the doubt. If adding the NOS terminator is enough for my transgen to finish transcribing correctly. So, does the...

26 December 2016 9,154 2 View