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Questions related from Erwin Luis Muñoz Acuña
Hi I have a question, I was reading some articles about different conditions for storing aliquots of antibiotics for use in culture media. But I would like to ask you, according to your personal...
28 August 2023 5,656 4 View
Hi all, I am doing assays with extracellular vesicles (EVs) in my lab and I have read in many articles that relate EVs concentration to protein concentration. For example, in this article...
30 November 2021 4,445 3 View
Hello, I would like to know why some vectors contain this AAV2 ITR sequence, I want to remove that sequence together with others of my vector to make the stable transfection with lipofectamine...
16 August 2018 7,589 4 View
Hello everyone, I have been reading about the addition of cap 5' in the maturation process of mRNA in eukaryotic cells and from what I have managed to understand, the addition of cap 5' is added...
01 May 2017 2,034 6 View
Hello everyone, I'm trying to transfect a bacterial operon to Arabidopsis thaliana. The operon consists of 6 genes and I am thinking of doing two constructos, one containing 4 and another one...
04 January 2017 5,443 2 View
Hello everyone, I am designing a "constructo" for Arabidopsis thaliana and I have the doubt. If adding the NOS terminator is enough for my transgen to finish transcribing correctly. So, does the...
26 December 2016 9,154 2 View