Erwin Luis Muñoz Acuña

8 Questions 6 Answers 0 Followers

Questions related from Erwin Luis Muñoz Acuña

Is it better to store antibiotics aliquots (in solution) at -80°C than at -20°C?

Hi I have a question, I was reading some articles about different conditions for storing aliquots of antibiotics for use in culture media. But I would like to ask you, according to your personal...

28 August 2023 5,705 4 View

Should I extract the proteins from extracellular vesicles (EVs) to correlate with the concentration of EVs in my sample, using Bradford's method?

Hi all, I am doing assays with extracellular vesicles (EVs) in my lab and I have read in many articles that relate EVs concentration to protein concentration. For example, in this article...

30 November 2021 4,490 3 View

Which is the AAV2 ITR sequence?

Hello, I would like to know why some vectors contain this AAV2 ITR sequence, I want to remove that sequence together with others of my vector to make the stable transfection with lipofectamine...

16 August 2018 7,650 4 View

Are my homology arms okay?

Hello everyone, I plan to use the CRISPR-Cas9 system in Arabidopsis thaliana and have designed 1600 kb long homology arms starting at 105 and 75 bs approximately from the cut site, The distance is...

22 May 2017 6,097 1 View

The addition of the cap 5' needs a signal sequence in the mRNA?

Hello everyone, I have been reading about the addition of cap 5' in the maturation process of mRNA in eukaryotic cells and from what I have managed to understand, the addition of cap 5' is added...

01 May 2017 2,079 6 View

The 35S CaMV promoter is able to give the signal to generate the Cap in mRNA of Arabidopsis?

Hello everyone, I needed to know if anyone knows this fact, or has had trouble expressing exogenous genes in Arabidopsis using the 35S CaMV promoter (2X35S more accurately) without putting a...

30 April 2017 8,134 5 View

Is the way in which I pose my constructon okay?

Hello everyone, I'm trying to transfect a bacterial operon to Arabidopsis thaliana. The operon consists of 6 genes and I am thinking of doing two constructos, one containing 4 and another one...

04 January 2017 5,483 2 View

Is the NOS terminator enough?

Hello everyone, I am designing a "constructo" for Arabidopsis thaliana and I have the doubt. If adding the NOS terminator is enough for my transgen to finish transcribing correctly. So, does the...

26 December 2016 9,182 2 View