Which solvent can be used for dispersion of Fe3O4 nanoparticle to determine antimicrobial activity against gram negative and positive bacteria?

I have chemically synthesised spherical shaped iron oxide nanoparticle bearing a size of 20-25 nm with zeta potential value of -34.6mV in aqueous solution. While performing antimicrobial assay, we...

05 June 2018 8,361 4 View

Protocol for dendritic cell isolation?

I would like to isolate dendritic cell from tissues of carps, but the chemicals (Nycoprep and Nycodenz) required for isolation is not available. I would be highly grateful if I could get any...

09 October 2017 1,931 1 View

Protocol for Dendritic Cell isolation?

03 April 2017 8,553 0 View

Purification of band from SDS-PAGE ?

If I do a dialysis with the help of a dialysis membrane of my protein and then perform SDS-PAGE to check my desired band...... Excise it and purify... Can I do the sequencing of it??? As I am...

02 March 2017 6,339 4 View

Query regarding RACE?

I am performing RACE PCR and in experiment of 5' and 3' PCR with the UPM and gene specific primer.... I m getting numerous band. Normally we should not get any band in the gel. I do have isolated...

10 November 2016 7,203 7 View

The ct value of housekeeping gene varies?

06 July 2016 1,125 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

How to predict the reaction pathway for electro organic synthesis?

Can anyone recommend some any software/models available that can predict substance reactivities and the reaction yield under a specific set of reaction conditions process and proposing pathways to...

17 February 2021 2,383 3 View