Which solvent can be used for dispersion of Fe3O4 nanoparticle to determine antimicrobial activity against gram negative and positive bacteria?

I have chemically synthesised spherical shaped iron oxide nanoparticle bearing a size of 20-25 nm with zeta potential value of -34.6mV in aqueous solution. While performing antimicrobial assay, we...

05 June 2018 8,361 4 View

Protocol for Dendritic Cell isolation?

03 April 2017 8,553 0 View

Purification of band from SDS-PAGE ?

If I do a dialysis with the help of a dialysis membrane of my protein and then perform SDS-PAGE to check my desired band...... Excise it and purify... Can I do the sequencing of it??? As I am...

02 March 2017 6,339 4 View

Query regarding RACE?

I am performing RACE PCR and in experiment of 5' and 3' PCR with the UPM and gene specific primer.... I m getting numerous band. Normally we should not get any band in the gel. I do have isolated...

10 November 2016 7,203 7 View

The ct value of housekeeping gene varies?

06 July 2016 1,125 3 View

Techniques used to bind DNA with a chemical???

What are the techniques required to bind DNA with a chemical.... and what are the properties and characteristics needed to be taken into considerations regarding the chemical.

11 December 2014 5,682 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View