Which solvent can be used for dispersion of Fe3O4 nanoparticle to determine antimicrobial activity against gram negative and positive bacteria?

I have chemically synthesised spherical shaped iron oxide nanoparticle bearing a size of 20-25 nm with zeta potential value of -34.6mV in aqueous solution. While performing antimicrobial assay, we...

05 June 2018 8,361 4 View

Protocol for dendritic cell isolation?

I would like to isolate dendritic cell from tissues of carps, but the chemicals (Nycoprep and Nycodenz) required for isolation is not available. I would be highly grateful if I could get any...

09 October 2017 1,931 1 View

Purification of band from SDS-PAGE ?

If I do a dialysis with the help of a dialysis membrane of my protein and then perform SDS-PAGE to check my desired band...... Excise it and purify... Can I do the sequencing of it??? As I am...

02 March 2017 6,339 4 View

Query regarding RACE?

I am performing RACE PCR and in experiment of 5' and 3' PCR with the UPM and gene specific primer.... I m getting numerous band. Normally we should not get any band in the gel. I do have isolated...

10 November 2016 7,203 7 View

The ct value of housekeeping gene varies?

06 July 2016 1,125 3 View

Techniques used to bind DNA with a chemical???

What are the techniques required to bind DNA with a chemical.... and what are the properties and characteristics needed to be taken into considerations regarding the chemical.

11 December 2014 5,682 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View