Hi everyone,
I'm currently have in culture the TE.354.T cell line (skin basal carcinoma) supplied by ATCC. These cells are maintained in DMEM supplemented with 10% FCS , L-Glutamine and antibiotics (Penicilin/Streptomycine) according to ATCC's handling informations.
These cells grow very slowly, but with their normal morphology. According to litterature, doubling time should be around 48h, but it seems that mine are slower.
Did some of you encountered the same problem?.
Thanks in advance for any informations and/or suggestions
Best regards
Hello Jean-Gregoire Marin
I have not handled TE.354.T cell line. Nevertheless, I would suggest that you modify your growth medium. In addition to 2mM L-Glutamine, you may add 1mM Sodium pyruvate and increase the serum concentration to 15%. Also, addition of fibroblast growth factor (FGF-2) (40-50ng/ml) may help.
Adding stromal derived factor-1 (SDF-1) (100 ng/mL) might also help. Please refer to the article attached below for more information.
Article Dermal fibroblast expression of stromal cell-derived factor-...
Best.
Hello Jean-Gregoire Marin
I also have in culture the TE.354.T cell line supplied by ATCC.
I maintained these cells in DMEM supplemented with 20% FCS and antibiotics (Penicilin/Streptomycine) according to ATCC.
I don't supplemented with L-Glutamine.
Like you, the cells I have take longer to grow than the time indicated by the ATCC.
I've tried to improve proliferation with other growth agents ((FGF-2, 50ng/ml) and (SDF-1, 100 ng/mL)) but without any real success unfortunately...
I hope it works out better for you.
In the meantime, have you been able to improve your cell growth?
Thanks in advance for any informations and/or suggestions
Best regards
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