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03 March 2021 5,360 2 View

With or without a Control Group?

Hello, I will do a research with K-12 students (my own students), and it includes a treatment which will last around 3 months. We will see how and whether it will affect their motivation. That's...

08 February 2021 632 4 View

Can I add a neutral option in a 4-point Likert scale?

Hello, I want to use a motivation scale with my students (secondary school) for my research, but the scale is in a 4-point Likert style with no neutral option (Certainly true - True - Not True -...

04 February 2021 4,757 13 View

What if there is no population to pilot an adapted questionnaire? What about its reliability and validity?

Hello, I'm new to this platform, and this is my first ever post in here. So, please excuse me in advance if I happen to cause any inconvenience. Coming back the question; I have just adapted a...

26 January 2021 9,030 4 View

Why are spectrophotometric absorbance values much higher than the initial value after electrochemical oxidation of organic matter?

After electrooxidation of an organic material, I get an excessive increase in absorbance values in wavelength scanning. What could be the reason for this? Can Radical formation cause...

20 January 2021 3,156 3 View

It is possible bulk density decrease with depth ?

Hi, It is possible bulk density decrease with depth ? (0-15 and 15-30 cm)

09 June 2020 4,951 15 View

Abaqus results of displacement?

Hi I make blast analysis using abaqus. I examined node displacement. I saw oscillation on displacement time history. But I don't understand whether the displacement is elastic or plastic.How can I...

30 January 2020 4,058 5 View

AMD in NAMD or flooding in GROMACS?

Which software would you recommend for aMD? Gromacs or NAMD? Also, what do you think about Gaussian aMD implementation in NAMD? Is it preferable to others?

29 October 2019 2,981 2 View

How using Agilent FieldFox VNA as a signal generator?

I want to take output power from the VNA to feed antennas? But I' m not using VNA as a signal generator.

20 February 2019 5,009 3 View

Why is the antenna designed for a single frequency oscillate at multiple frequencies?

Although we determine the size of the microstrip antenna for a single frequency, why it seems to have a resonance frequency on more than one frequency? Is there an antenna function at each...

13 February 2019 5,064 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View