If you were to analyse metabolic pathways in T cells (murine and human), how would you prepare the culture medium? And, apart from classical activation, would you add IL-2 for a 3-5 day cell culture?
Dear Michelangelo Certo ,
We use RPMI1640 (supplemented with 10% FBS and antibiotics) for our lymphocyte culture. Now we purchase ready made media from Gibco but earlier we used to purchase powered media and dissolve it in autoclaved distilled water followed by filtration. this is our incomplete media. Now, we used to complete this media by supplementing it with FBS and antibiotics before use.
we are culturing Lymphocytes and T cells upto 72 hours, but we do not use IL2 in culture. but it is recommended to add IL2 in culture for better health after 24 hours.
Tj Pl
As i have mentioned we don't use Il2 in our culture system, but i know few people who use IL2 in cultute and replenish it in 3-4 days at the concentration of 50 IU/mL.
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biomolecules
- Peptides