Hello, I try to find some literature about T cell and ADCC resistance. I wonder why people tell me that T cells are ADCC-resistant, where can I find relative research? Thanks.
Cuáles fueron las tendencias en investigaciones en arquitectura, urbanismo y patrimonio edificado en decadas del 2000 al 2020? Porque requiero conocer tesis de posgrado nivel maestría...
24 July 2024 5,494 1 View
Hello, I have been performing Western Blots to study the protein levels of a rodent skin and muscle sample. However, I have not been able to produce homogenous reference bands. I suspect that...
26 June 2024 5,913 3 View
Hi, I have a question regarding western blot. I performed total protein statin after trasfering membrane. The result was totally fine before (Figure 1). However, there were strange bubble-like...
30 May 2024 8,045 3 View
When refining with HighScore Plus, inserting the CIF file for gamma-Ca2SiO4 and then refining it will show "unusual space group setting for phase: gamma-Ca2SiO4". This occurs for all gamma-Ca2SiO4...
19 May 2024 10,033 1 View
The preprint papers on ResearchGate are not accessible/read by AI models such as ChatGPT and others. The papers are in image format but not PDF format. So the preprint papers on ResearchGate are...
17 May 2024 5,304 3 View
As the title described, it is common that a target gene sequence contains motif that acts as the binding site of transcription factor rather then the upstream regulatory region. Does this affect...
13 May 2024 1,099 3 View
As title describes, we're trying to mutate an amino acid of antibody Fc to cysteine, followed by TCEP to further drug conjugation, one of my colleague had raise the concern of purified TCEP...
29 April 2024 3,227 2 View
How can isolate C2C12 myotube efficiently from C2C12 myoblast?
18 April 2024 6,123 0 View
Hi, I am running a titration of QAC with sodium tetraphylborate using autotitrator from Metrohm. The titrator identifies multiple end point with different ERC value. Would like to know which EP...
17 April 2024 545 1 View
Blood transfusion is a critical medical procedure that can save lives in various situations. However, according to Chevalier et al. (2016), as stated in the article of Kavulavu et al. (2022)...
11 April 2024 7,391 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View