Can I use this method to prepare ZnO-Chitosan nanocomposite or not?

The CH-CuO nanoparticles were synthesized following a previously reported protocol (Maheo, 2022). A total of 50 mL of 0.05 M copper sulphate solution was mixed with 20 mL of leaf extract via continuous stirring for an hour. After stirring, the pH of the solution was then adjusted to 9 by adding 0.2 M NaOH dropwise. Meanwhile, chitosan solution was prepared by dissolving 20 mg of chitosan in 20 mL acetic acid solution (1.5%) under stirring at 60°C. A total of 20 mL of chitosan solution was then added to the plant extract–copper sulphate mixture, and the solution was then stirred continuously for 2 h at 60°C. The solution was centrifuged at 10,000 rpm for 20 min, and the nanoparticles at the bottom were filtered, washed with double-distilled water, and dried. For the biosynthesis of the CuO and chitosan (CH) nanoparticles, a similar protocol was followed, except for the addition of chitosan and copper sulphate solutions, respectively.