Hello everyone,
I just read papers about fluorescent gold nanoparticle synthesized from, for example, BSA, lysozyme, HRP for glucose detection, has anybody ever synthesized fluorescent gold nanoparticle directly from glucose oxidase?
Dear Diem Phuong Nguyen:
The glucose oxidase (GO) enzyme is an oxido-reductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. GO - catalyst, glucose - reducing agent, oxygen –oxidant. The catalyst lowers the activation energy of a redox reaction.
Au ion - oxidant, BSA - reductant. Perhaps GO have group -SH. Then GO may be a reducing agent
Best regards.
Dear Professor Mirgorod,
Thank you for your time to reply my question.
Actually, follow your direction, I tried to synthesize gold nanoparticle in glucose oxidase solution and got some fluorescence.
But I have some difficulty in purifying the gold nanoparticle, when I tried to filter gold-glucose oxidase complex using centrifuge tube with filter inside (10, 000 Da cutoff filter), it seems like it centrifuged out the nanoparticle (
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