I'm working with some precast BioRad gels (1% agarose, up to 96 well format) and want to boost sensitivity for single stranded DNA. Looks like BioRad has been discontinuing products, and the only precast gels available are with ethidium bromide. Has anyone tried to exclude EtBr from the running buffer and then do SYBR Gold staining afterwards? Any competition effects?
And just to provide a few details. Products will be produced from an enzymatic reaction, denatured with formamide (RNA loading buffer), 10 Kb to waste of $$$. And I could of course cast the gels myself, but I'm trying to only use QC'd, commercial reagents/supplies.
Thanks!