Dear Tanya!

Please You look at the dissertation:

Interrogating the Compositional and Functional Diversity of mSWI/SNF Complexes

Genome-scale fitness screening reveals cancer-specific dependencies on ncBAF complexes (page 41)

Figure 2.2. ncBAF complex components are selective synthetic lethal dependencies in synovial sarcoma and malignant rhabdoid tumor cell lines.

(C) Heatmap of dependency scores in SYO-1 (SS18-SSX-driven synovial sarcoma) and SW982 (histological synovial sarcoma mimic without SS18-SSX translocation) ranked by difference in dependency showing ncBAF-specific components are dependencies only in the SS18-SSX driven cell line

CRISPR/Cas9 mediated genome editing

Genome editing was performed using CRISPR/Cas9. Target-specific sgRNA sequences were chosen using the Benchling web application and The Broad Institute’s Genetic Perturbation Platform web application. These sgRNA sequences were cloned into the Px459 vector (Addgene). 5ug of CRISPR/Cas9 vector along with 50ng of pmaxGPF Vector (Lonza) was then nucleofected into ~1 million Jurkat cells using the X-05 Nucleofector I Device (Lonza). (page 102)

Hi Alexandr Chernov

Thank you for the reply but I can't seem to find anywhere in that dissertation a solution and pulse code used for the nucleofection of the cells...