ELISPOT normalization with varying cell numbers?

Hello everyone, due to a calculation error in the spreadsheet, the splenocytes I used were wrongly diluted. As a result, the number of cells per well are identical for each mouse, but differ...

15 December 2020 9,344 1 View

How do I structure my panel data in an event study?

Hey, I am conducting an event study with a short event window (lets say 3 or 5 days). I have a lot of variables, but most of them are fixed over that short period of time (GDP per capita, GDP...

11 November 2020 3,582 6 View

Jamovi - mean centred moderators?

Jamovi free ware has a built-in interaction function (a product term) in linear regression procedure. Does anybody know if Jamovi automatically mean centres variables included in the product...

04 August 2020 6,418 8 View

How to extract legal speed limit from GPS coordinates in OSM (OpenStreetMap) ?

I am interested in the extration of speed limit from Longitude and Latitude coordinate values. Is it possible to find speed limit attribute assigned to specific Open Street Map position and is...

30 March 2020 9,201 3 View

Human Isogenic H3.3K27M cell lines?

Hello all, I'm trying to identify pairs of isogenic human cell lines (preferably brain) in which the parent line does not possess the H3.3K27M mutation while the other one does. I've been finding...

26 March 2020 1,439 2 View

Has GPS detected the ethrer bwind?

Abstract An illusion of undecidability of an ether wind was the basis for establishing the theory of special relativity (SRT). The reason to that mistake was a wrong interpretation of light...

08 February 2020 6,990 4 View

Does source code language matter in a paper?

During literature review, I have noticed that many papers under the umbrella of software engineering use Java when code examples or small demonstrations are appropriate. I understand that Java is...

10 January 2020 1,763 7 View

How do we measure the concentration of two different complex in the same solution: Rh2(SO4)3 and RhCl3?

Hi all. I have solution containing mixture of two complexes of rhodium Rh2(SO4)3 and RhCl3, the concentration about 100 ppb - 2 ppm each with matrix SO4 2- and Cl- about 2 M, is there any...

11 November 2019 5,753 1 View

Does anyone know a reliable dataset of wave height in different locations of the Mediterranean Sea?

Does anyone know a reliable dataset of wave height in different locations of the Mediterranean Sea?

14 May 2019 6,318 4 View

Is saturation a tool of qualitative research that works across all epistemological perspectives?

Saturation is the approach to collecting qualitative data until you reach a point where you are not getting any new details. It seems this tool would work across most if not all epistemological...

18 March 2019 8,890 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View