Suggest me a best DNA sequencing facility in USA for sequencing a gene fragment cloned in a plasmid?

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Planting density for walnut timber production in forestry plantation?

For timber production in forestry plantation, what is the best planting density for Juglans regia and J. nigra (Black walnut) for minimising lateral branch growth. In the UK forestry, this species...

21 July 2024 8,799 4 View

Can I use CCCP instead of H₂O₂ as a positive control for ROS measurement through flow cytometry?

I am giving starvation stress to the soil amoeba (Dictyostelium discoideum) cells in my study groups and using NAC as a negative control. I am using DHE for ROS measurement.

20 July 2024 6,354 0 View

Can a paper that is posted as a preprint in a database with DOI, can be reviewed in another journal and accepted for publication??

It happened that I am asked to review a paper for a journal. Incidentally, the paper is posted as a preprint in another database as open access with DOI. It is completely citable. Now, should I...

17 July 2024 1,831 4 View

How to troubleshoot the Milliplex Cytokine assay, when I see >OOR/<OOR in my data?

I am conducting cytokine measurements using a multiplex assay. Some of the proteins are yielding results that fall outside the standard curve values, either below (< OOR) or above (> OOR)....

24 June 2024 9,717 0 View

Where to perform invitro transcription and capping?

I am planning to do invitro transcription and capping for an mRNA vaccine generation. Can anyone suggest whether it should be done on a bench top or inside a laminar hood?

14 May 2024 186 3 View

How important are Border Studies in present time?

Borders are markers of nations. Do cartographical decisions involve emotions especially partitioning a unified territorial region ?

29 April 2024 3,715 5 View

What all parameter can affect FcgRI binding of a IgG4 MAB?

What all parameter play role in binding of IgG4 Mab to FcgRI?

05 March 2024 5,103 0 View

What is the median discharge voltage for any energy storage device?

04 February 2024 8,068 1 View

Why the colombic efficiency of supercapacitor device initial decreases then increase for 1000 cycles of charge discharge?

why the colombic efficiency of supercapacitor device initial decreases then increase for 1000 cycles of charge discharge?

03 February 2024 281 2 View

What is the minimum light requirement during early growth (sapling stage) for under canopy species?

For under canopy planting, what is the standard minimum requirement of light quantity reaching through created gaps for the early growth of understory species; I do understand that the light...

22 January 2024 700 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View